兔抗PRKACA(Phospho-Thr197)多克隆抗体

Background:

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

Applications:

WB, IHC, IF

Name of antibody:

PRKACA(Phospho-Thr197)

Immunogen:

Peptide sequence around phosphorylation site of threonine 197(R-T-W(p)-T-L) derived from Human PKA α/β CAT.

Full name:

protein kinase, cAMP-dependent, catalytic, alpha

Synonyms：

PKACA

SwissProt:

P17612

IHC positive control:

Human colon carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

41 kDa

WB Positive control:

Mouse brain tissue lysates

WB Recommended dilution:

500-1000

IF positive control:

A549 cells

IF Recommend dilution:

100-200