小鼠抗SF1单克隆抗体

Background:

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Applications:

WB

Name of antibody:

SF1

Immunogen:

Fusion protein of human SF1

Full name:

splicing factor 1

Synonyms:

BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25

SwissProt:

Q15637

WB Predicted band size:

68 KD

WB Positive control:

NCI-H460, Hela, PC3, MCF7, A549 cell lysates

WB Recommended dilution:

500-2000